Licensing Biomedical Informatics and Research Administration are working together to provide ImageQuant TL software from GE Healthcare Life Sciences for research image analysis. This software was purchased for use with the GE Life Sciences Typhoon 9500FLA digital imager located in R3.302, but can also be used to analyze images from other sources.

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Imagequant Las 500 - Cytiva Life Sciences - 29005063

Marca: Cytiva

EQUIPAMENTO FOTODOCUMENTADOR PARA CAPTURA DE IMAGENS DE WESTERN BLOTTINGQUIMIOLUMINESCENTE, COLORIMETRICO E UV FLUORESCENTE

GE ImageQuant TL Image Analysis Software Set IQTL8.1 and IQTLSecurlty8.1 29000605 Picture is from real stock. New and Good physical condition. Please contact us for more information or pictures. Location: Shanghai 201801, China. Deliver the most from your GE Digital software allowing you to focus on your business needs. Explore Services Industries Electric Utilities Services Explore Services. What You Get: GE Digital’s expert service and support teams create value and deliver on business objectives. Imaging Application handbooks Installing ImageQuant TL Software The Department has purchased a site-license for the iQTL software. Cchmc Imaging Core For its use, one needs to download the client software from GE Healthcare (Windows compatible), and point the program to the license on our Departmental server.

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Marca: Cytiva

EQUIPAMENTO FOTODOCUMENTADOR PARA CAPTURA DE IMAGENS DE WESTERN BLOTTINGQUIMIOLUMINESCENTE, COLORIMETRICO E UV FLUORESCENTE

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Descrição do Produto

ImageQuant LAS 500 is a cooled CCD camera for chemiluminescent Western blot imaging, blue/UV based DNA & protein stains, and white-light imaging of colorimetric stains and markers.

• Sensitivity: detect picogram levels of sample with chemiluminescence.
• Compact integrated design: built-in touchscreen lets you work independently of a dedicated PC, has a small footprint that fits neatly on any lab bench.
• Automatic overlay function: simultaneously image and overlay chemiluminescent samples and colored molecular weight markers, making molecular weight determination easy.
• Flexible image storage: store images on a USB memory stick, on the system, or in a network connection folder.
• Simplicity: auto, semi-auto, and increment exposure modes with a user-friendly touchscreen operation helps you take the best possible image.
• Speed: ready to operate in less than five minutes with the rapid cooling CCD camera.
• Ease of installation: just unpack, power up and start.

Designed for chemiluminescence detection and gel documentation

This CCD imager is a practical and affordable solution to individual researchers or laboratories that are either looking for their own imager or need extra imaging capacity. The 30 × 28 cm footprint has the same area as a typical laptop computer, which allows the imager to fit neatly on almost any lab bench without the need to connect ancillary equipment.

Ready for use in less than five minutes

ImageQuant LAS 500 imager consists of a touchscreen, sample chamber, USB port, camera and light sources. The 8.3-megapixel CCD camera is ready for use in less than five minutes. Reliable Peltier cooling combined with binning significantly reduces noise levels and improves both the sensitivity and linearity of the system.

Flexible imaging modes for Optimal chemiluminescence detection

Signal intensity can be detected either automatically or semi-automatically by specifying an area of interest. In addition, single (manual) or multiple (incremental) exposures can be set by the operator. The camera is designed for optimal chemiluminescence detection with regard to sensitivity and dynamic range.

Three LED light sources

The system features three LED light sources: a combined blue epi-/UV epi-light and a white epi-light. The combined blue epi-/UV epi-light allows imaging of fluorescence signals above 550 nm. This feature makes the camera well suited for DNA stain imaging with SYBR™ Green, ethidium bromide, or Deep Purple Total Protein Stain.

A fast and easy way to verify the molecular weight of detected protein bands

The white epi-light source (470 to 635 nm) enables documentation of colorimetric markers and stains, such as Coomassie Blue or Silver. Moreover, the white light mode can be combined with the chemiluminescence mode to automatically generate an overlay image of the colored marker and chemiluminescent sample images. This provides a fast and easy way to verify the molecular weight of detected bands, and identification of band of interest if multiple bands are generated.

Software

In addition to the on-board software, images acquired using ImageQuant LAS 500 can be analyzed using ImageQuant TL (IQTL). IQTL is an automated and easy-to-use general-purpose gel, blot, and array image analysis software. IQTL requires installation on an external computer and cannot be installed on ImageQuant LAS 500.

Imagequant Tl Iqtl Software Ge Healthcare System

Western blot detection

Western blotting, also known as immunoblotting, or protein blotting, is a well-established and widely used technique for the detection and analysis of proteins. A variety of detection systems, based on chemiluminescence, chemifluorescence, fluorescence, chromogenic, or radioisotopic detection are available. Chemiluminescence is reliable and well known for having the greatest sensitivity of any available detection method.

Further, there has been a decline in usage of radioisotopic and chromogenic reagents due to safety issues with handling radioactive isotopes and poor sensitivity with chromogenic reagents, which has meant, enzyme-based chemiluminescent and direct fluorescence have been extensively developed and are now the preferred method for detection due to their improved sensitivity and wider dynamic range.

What is chemiluminescence western blot detection?

Imagequant Tl (iqtl) Software (ge Healthcare)

Chemiluminescence Western Blot detection is based on antibodies conjugated to horseradish peroxidase (HRP) that catalyze the oxidation of luminol in presence of peroxide, resulting in light emission. HRP has several advantages over other enzymes such as alkaline phosphatase (AP). HRP can be easily conjugated to antibodies or streptavidin (which binds with extraordinarily high affinity to biotin, a commonly used tag, and can be used with different chemiluminescent reagents. Considerable efforts have been made to develop HRP-based detection reagents to obtain higher detection sensitivity, stronger light intensity and long-lasting signals.

Basic Information

ImageQuant TL is a suite of image analysis tools designed to meet a broad range of analysis requirements. ImageQuant TL is an automated and easy-to-use general purpose gel, blot, and array image analysis software.

The flexible ImageQuant TL software features high levels of automation, yet still allows manual editing at any stage of the analysis. Image data can be extracted easily and accurately in a matter of seconds to produce consistently reliable data. ImageQuant TL software also supports multiplexing for advanced fluorescence applications.

Licensing

Biomedical Informatics and Research Administration are working together to provide ImageQuant TL software from GE Healthcare Life Sciences for research image analysis. This software was purchased for use with the GE Life Sciences Typhoon 9500FLA digital imager located in R3.302, but can also be used to analyze images from other sources. IQTL will primarily be used to analyze gels of various types, but can also be used for:

- 1-D electrophoresis gels;

- dot blots, microplates, and basic arrays

- colony counting and measurement of 2-D spots and other image features

IQTL will be provided through a floating license pool administered by BMI. There is no charge to download or use the software at this time, and no chargebacks are expected in the future. There should be enough floating licenses for the institution, but we will purchase more if the current number reaches capacity.

Availability:

Cincinnati Children's employees only.

Compatibility:

Will run on MS Windows-based computers only. Mac users can request to run it via a Citrix server OR on a virtual machine (e.g.,using Parallels or VMWare).

Cost to Investigators:

None.

To Install

Imagequant Tl Iqtl Software Ge Healthcare Login

To have IQTL installed on your computer, please send an email to ResearchEquipment@cchmc.org with

* Your name

* PI name

* Operating System of the machine (Windows or Mac)

* Asset tag number of the machine on which IQTL is to be installed

(if installing on a Mac, provide the asset tag # of the VM and NOT the Mac)

IS will be notified to push out the software to your computer, and you will receive an email with the license server name (you will need this to set IQTL up the first time you run it). You will also be included in the user email list.

User List

Research Administration will maintain a user mailing list in order to inform users about important future changes in the software, ie. new versions, upgrades, issues, etc.

More Information

IQTL Imaging Principles Handbook – extensive resource for imaging analysis from GE Healthcare

For questions about the Typhoon, other imaging equipment, the user list, or software downloads, please contact ResearchEquipment@cchmc.org

For technical issues with using floating licenses, please contact help@bmi.cchmc.org